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World Health Organization : Year 1985-86 ; World Health Organization Brn 6676, (Part 2): Appendix 3 ; A Programme for the Provision of Matched Assay Reagents for the Radioimmunoassay of Hormones in Reproductive Physiology

By Special Programme of Research

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Book Id: WPLBN0000058061
Format Type: PDF eBook
File Size: 3.0 MB
Reproduction Date: 2005

Title: World Health Organization : Year 1985-86 ; World Health Organization Brn 6676, (Part 2): Appendix 3 ; A Programme for the Provision of Matched Assay Reagents for the Radioimmunoassay of Hormones in Reproductive Physiology  
Author: Special Programme of Research
Volume:
Language: English
Subject: Health., Public health, Wellness programs
Collections: Medical Library Collection, World Health Collection
Historic
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Publisher: World Health Organization

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Of Research, Development And Research Training In Human Reproductio, S. P. (n.d.). World Health Organization : Year 1985-86 ; World Health Organization Brn 6676, (Part 2). Retrieved from http://cn.ebooklibrary.org/


Description
Medical Reference Publication

Excerpt
Radioimmunoassay (RIA) has been used since its introduction in the late 1950s for the measurement of steroid and polypeptide hormones as well as many other substances of biological interest. The use of this analytical technique has rapidly spread in all branches of biomedical science. body-binding sites so that the greater the amount of unlabelled hormone the less is the amount of tracer bound to the antibody. The concentration of the hormone in the unknown sample is obtained by comparing the amount of tracer bound to antibody with the values obtained for standard solutions of the hormone.

Table of Contents
CONTENTS 1. GENERAL INTRODUCTION Radioimmunoassay : general principles WHO Matched Reagent Program Use of reagents Use of pipettes Use of tubes Use of counters Use of result sheet Calculation of results Quality control Use of SI units 2. ASSAY OF STEROID HORMONES 2.1 Preparation, storage and use of reagents 2.1 .I Assay buffer 2.1.2 Charcoal suspension 2.1.3 Steroid standards 2.1.4 Steroid antisera 2.1.5 Steroid tracers 2.1.6 Counting solution 2.2 General assay procedure for progesterone, testosterone and estradiol 2.2.1 Sampling procedure 2.2.2 Recovery Corrections 2.2.3 Extraction and evaporation 2.2.4 Redissolving and aliquoting 2.2.5 Assay procedure 2.2.6 Charcoal separation 2.2.7 Counting


 
 



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