QR link for The Subtle Effects of Sea Water Acidification on the Amphipod Gammarus Locusta : Volume 6, Issue 1 (15/01/2009)
Add to Book Shelf
Flag as Inappropriate
Email this Book

The Subtle Effects of Sea Water Acidification on the Amphipod Gammarus Locusta : Volume 6, Issue 1 (15/01/2009)

By Hauton, C.

Click here to view

Book Id: WPLBN0004005387
Format Type: PDF Article :
File Size: Pages 28
Reproduction Date: 2015

Title: The Subtle Effects of Sea Water Acidification on the Amphipod Gammarus Locusta : Volume 6, Issue 1 (15/01/2009)  
Author: Hauton, C.
Volume: Vol. 6, Issue 1
Language: English
Subject: Science, Biogeosciences, Discussions
Collections: Periodicals: Journal and Magazine Collection (Contemporary), Copernicus GmbH
Publication Date:
Publisher: Copernicus Gmbh, Göttingen, Germany
Member Page: Copernicus Publications


APA MLA Chicago

Hauton, C., Williams, J., & Tyrrell, T. (2009). The Subtle Effects of Sea Water Acidification on the Amphipod Gammarus Locusta : Volume 6, Issue 1 (15/01/2009). Retrieved from http://cn.ebooklibrary.org/

Description: School of Ocean and Earth Science, University of Southampton, National Oceanography Centre, European Way, Southampton, Hants, SO14 3ZH, UK. We report an investigation of the effects of increases in pCO2 on the growth and molecular physiology of the neritic amphipod Gammarus locusta, which has a cosmopolitan distribution in estuaries. Amphipods were reared from juvenile to mature adult in laboratory microcosms at three different levels of pH in nominal range 8.1–7.6. Growth rate was estimated from weekly measures of body length. At sexual maturity the amphipods were sacrificed and assayed for changes in the expression of genes coding for a heat shock protein (hsp70 gene) and the metabolic enzyme glyceraldehyde-3-phosphate dehydrogenase (gapdh gene). The data show that the growth and survival rate of this species is not significantly impacted by a decrease in sea water pH of up to 0.5 units. Quantitative real-time PCR analysis indicated that there was no significant effect of growth in acidified sea water on the expression of the hsp70 gene. However, there was a consistent and significant increase in the expression of the gapdh gene at a pH of ~7.5 which indicated a possible disruption to oxidative metabolic processes. It was concluded that future predicted changes in sea water pH may have subtle effects on the physiology and metabolism of coastal and marine species which may be overlooked in studies of whole organism response.

The subtle effects of sea water acidification on the amphipod Gammarus locusta

Borg,~I., Rohde,~G., Löseke,~S., Bittscheidt,~J., Schultze-Werninghaus,~G., and Bufe,~S A.: Evaluation of a~quantitative real-time PCR for the detection of respiratory syncytial virus in pulmonary diseases, Eur. Resp. J., 21, 944–951, 2003.; Bustin,~S A.: Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays, J. Mol. Endocrin., 25, 169–193, 2000.; Al-Rasheid,~K A S. and Sleigh,~M A.: Distribution and abundance of interstitial ciliates in Southampton Water in relation to physicochemical conditions, metal pollution and the availability of food organisms, Est. Coast. Shelf Sci., 41, 61–80, 1995.; Altschul,~S F., Madden,~T L., Schaffer,~A A., Zhang,~J H., Zhang,~Z., Miller,~W., and Lipman, D. J.: Gapped BLAST and PSI-BLAST: a~new generation of protein database search programs, Nuc. Acids Res., 25, 3389–3402, 1997.; Attrill,~M J., Power,~M., and Thomas~R M.: Modelling estuarine Crustacea population fluctuations in response to physico-chemical trends, Mar. Ecol. Prog. Ser., 178, 89–99, 1999.; Berge,~J A., Bjerkeng,~B., Pettersen,~O., Schaanning,~T., and Øxnevad,~S.: Effects of increased sea water concentrations of \chemCO_2 on growth of the bivalve \textitMytilus edulis~L, Chemosphere, 62, 681–687, 2006.; Bustin,~S A.: A~to Z of Quantitative PCR. IUL Biotechnology Series. International University Line, La Jolla California, p 882, 2004.; Caldeira,~K. and Wickett,~M E.: Ocean model predictions of chemiostry changes from carbon dioxide emissions to the atmosphere and ocean, J. Geophys. Res., 110, C09S04, 2005.; Charmantier Daures~M. and Vernet,~G.: Moulting, autotomy and regeneration. pp. 161–255, in: The Crustacea, edited by: Forest,~J. and von Vaupel Klein~J C., Volume 1. Brill Publishers, Leiden, 2004.; Dalhoff,~E P.: Biochemical indicators of stress and. metabolism: applications for marine ecological studies, Annu. Rev. Physiol., 66, 183–207, 2004.; Daugaard~M., Rohde,~M., and Jaattela~M.: The heat shock protein 70 family: highly homologous proteins with overlapping and distinct functions, FEBS Lett., 581, 3702–3710, 2007.; Dupont,~S., Havenhand,~J., and Thorndyke,~M.: \chemCO_2-driven acidification radically affects larval survival and development in marine organisms, Comp. Biochem. Physiol., 150A, S170–S170, 2008.; Factor,~J R.: Biology of the Lobster. Academic Press, San Diego, p 528, 1995.; Gazeau,~F., Quiblier,~C., Jansen~J M., Gattuso, J-P., Middleburg,~J J., and Heip,~C H R.: Impact of elevated \chemCO_2 on shellfish calcification, Geophys. Res. Lett., 34, L07603, doi:10.1029/2006GL028554, 2007.; Hall-Spencer,~J M., Rodolfo-Metalpa,~R., Martin,~S., Ransome,~E., Fine,~M., Turner,~S M., Rowley,~S J., Tedesco,~D., and Buia,~M.-C.: Volcanic carbon dioxide vents show ecosystem effects of ocean acidification, Nature, 454, 96–99, 2008.; Hayward,~P J. and Ryland,~J S.: Handbook of the Marine Fauna of North-West Europe, Oxford University Press, Oxford, p 812, 1995.; Higgins,~D G. and Sharp,~P M.: CLUSTAL: a~package for performing multiple sequence alignment on a~microcomputer, Gene, 73, 237–244, 1988.; Karlin,~S. and Brocchieri,~L.: Heat shock protein 70 family: multiple sequences comparisons, function and evolution,~J. Mol. Evol., 47, 565–577, 1998.; Houghton,~J T., Ding,~Y., Griggs,~D J., Noguer,~M., van der Linden,~M., Dai, ~X., Maskell,~K., and Johnson,~C A.: Climate Change 2001: the Scientific Basis (Contribution of WG1 to the IPCC Third Assessment) Cambridge, Cambridge University Press, 2001.; Kikkawa,~T., Kita,~J., and Ishimatsu,~A.: Comparison of lethal effect of \chemCO_2 and acidification on red sea bream (\textitPagrus major) during the early developmental stages, Mar. Poll. Bull., 48, 108–110, 2004.; Kleypas,~J A., Buddemeier,~R W., Archer,~D., Gattuso,~J P., Langdon,~C., and Opdyke,~B N.: Geochemical consequences of increased atmospheric carbon dioxide on coral reefs, Science, 284, 118–120, 1999.; Kleypas,~J A., Feely,~R A., Fabry,~C., Langdon,~C L., Sabine~C L., and Robb


Copyright © World Library Foundation. All rights reserved. eBooks from World eBook Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.